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The Body Wall and Musculature of the Marine Triclad Palombiella stephensoni (Palombi, 1938): Part One: General Tissue Structure as seen with the Light Microscope

[Plates]

page 8

Plate 1

page 9
Plate 1. The Epidermis

Plate 1. The Epidermis

Fig. 1 Transverse section of the dorsal epidermis stained by the Falg technique and photographed by Nomarski interference microscopy. The nuclei of the cells are large oval structures. The outer 1/3 region of the cells possesses a system of internal "channels" which are perpendicular to the base of the cells.

i.m., region of internal channels; n., nuclei; p., subepidermal pigment; pa., parenchyma.

Fig. 2 Photomicrograph of a portion of dorsal epidermis sectioned tangentially. The section is at a level corresponding to A-B in Fig. 1, and passes through the bases of epidermal cells. Connective fibres enclose the cell bases, giving a polygonal pattern, in which each cell is mostly surrounded by 6 other cells. Stained by Mallory's technique.

cf., connective fibres; cm., cellular material.

Fig. 3 Photomicrograph of a portion of dorsal epidermis sectioned tangentially. The section is at a level corresponding to C-D in Fig. 1. The system of internal "channels" is cut transversely and appears as many small tubes. Secretions from 3 subepidermal eosinophil glands are present. Stained by Mallory's technique and photographed by Nomarski interference microscopy.

im., the region of internal "channels" cut transversely; es., secretion from eosinophil glands.

page 10

Plate 2

page 11
Plate 2. The Basement Membrane, Muscles, and subepidermal glands

Plate 2. The Basement Membrane, Muscles, and subepidermal glands

Fig. 1 Sagittal section of dorsal body wall. The "basement membrane" is thick, densely stained and sends "septa" between epidermal cells so that the cells sit in cup-like depressions in the "basement membrane". Mallory Stain.

bm., basement membrane; d., dorsoventral muscle fibres; e., epidermis; pa., parenchyma; s., septum.

Fig. 2 Sagittal section of ventral body wall. The "basement membrane" is a thick, dense line. Circular muscle fibres are separated by fine connective fibres which are continuous with the "basement membrane". Mallory stain.

bm., basement membrane; cm., circular muscle fibres; cf., connective fibres; d., dorso-ventral muscle fibres; e., epidermis; lm., longitudinal muscle fibres; pa., parenchyma.

Fig. 3 Sagittal section of anterior region of the parenchyma. Basiphil and eosinophil glands are present. Both types are long and slender. Mallory stain.

bg., basiphil glands; eg., eosinophil glands; e., epidermis; pa., parenchyma.

page 12

Plate 3

page 13
Plate 3. The Eosinophil Glands

Plate 3. The Eosinophil Glands

Fig. 1 Transverse section of the dorso-lateral epidermis. Eosinophil secretions within the parenchyma are entering the bases of 2 epidermal cells. At the surface of the epidermal cells is a row of "papillae". Stained with Delafield's haematoxylin and eosin, photographed by Nomarski interference microscopy.

es., eosinophil secretion; n., nuclei of epidermal cells; pa., parenchyma; p., papillae.

Fig. 2 Transverse section showing subepidermal eosinophil glands opening to the ventro-lateral surface. The secretions reach the surface by passing through epidermal cells. Fan-shaped systems of ducts are formed within the epidermal cells. "Papillae" are seen where secretions reach the surface. Stained with the Falg technique and photographed by Nomarski interference microscopy.

e., epidermis; es., eosinophil secretion; p., papillae; pa., parenchyma.

Fig. 3 Tangential section of epidermis at a region where eosinophil secretions reach the surface. The section is at a level corresponding to C-D in Plate 1, Fig. 1. The arrangement of the secretion masses is evidence that the secretions actually pierce epidermal cells, and do not reach the surface by penetrating between epidermal cells. Falg staining, photographed by Nomarski interference microscopy.

es., eosinophil secretion; im., region of internal channels of epidermis.

Fig. 4 Tangential section of epidermis at a level corresponding to A-B in Plate 1, Fig. 1. The polygonal patern of cells is apparent. One cell (arrowed) contains large eosinophil secretory ducts from underlying glands. Stained and photographed as for Fig. 3.

p., pigment.