B. Hypophasic Pigments

The bottom layer of the extract when developed on a thin layer chromatogram showed three faint bands which decolorised very rapidly. (The solvent used was five parts petroleum ether to one part methanol). Nevertheless, one can distinguish three layers which did not move very far from the initial spot. This is to be expected page 4 page 5 because the mixture of pigments obtained was extracted in the aqueous-alcoholic layer. The three bands in ascending order are:

• Band G — greenish-yellow
• Band H — also greenish-yellow
• Band I — yellow