1. | Lilie's triple stain of Weigert's haematoxylin, aqueous eosin y, naphthol green B is unreliable as a differential stain for connective tissue. |
2. | The main fault with the method is rapid loss of eosin from sections during dehydration. |
3. | Excellent differential connective tissue staining can be obtained using a plasma stain of eosin in 90% alcohol with the connective tissue stain in a clearing agent or in cellosolve. |
4. | The recommended technique employs Delafield's haematoxylin as nuclear stain, eosin y in 90% alcohol as plasma stain, and fast green FCF in clove oil or in cellosolve as the connective tissue stain. |