Other formats

    Adobe Portable Document Format file (facsimile images)   TEI XML file   ePub eBook file  

Connect

    mail icontwitter iconBlogspot iconrss icon

Zoology Publications from Victoria University of Wellington—Nos. 42 to 46

Summary

Summary

1.Lilie's triple stain of Weigert's haematoxylin, aqueous eosin y, naphthol green B is unreliable as a differential stain for connective tissue.
2.The main fault with the method is rapid loss of eosin from sections during dehydration.
3.Excellent differential connective tissue staining can be obtained using a plasma stain of eosin in 90% alcohol with the connective tissue stain in a clearing agent or in cellosolve.
4.The recommended technique employs Delafield's haematoxylin as nuclear stain, eosin y in 90% alcohol as plasma stain, and fast green FCF in clove oil or in cellosolve as the connective tissue stain.